A decrease absorbance after addition of the antioxidant was a measure of its superoxide scavenging activity. 2021 Jul 15;19(1):102. doi: 10.1186/s43141-021-00203-5. Bokhary KA, Maqsood F, Amina M, Aldarwesh A, Mofty HK, Al-Yousef HM. However, a non-significant correlation was observed between the ABTS, OH scavenging activity and the total phenolic and flavonoid content of these extracts. EEF: ethyl acetate fraction, BuF: n-butanol fraction. Khled khoudja N, Boulekbache-Makhlouf L, Madani K. Antioxidant capacity of crude extracts and their solvent fractions of selected Algerian Lamiaceae. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. official website and that any information you provide is encrypted Each sample was measured in triplicate and averaged. In vitro antioxidant activity of roots of Thespesia lampas Dalz and Gibs. When pretreated of EEF or BuF at concentration of 500g/mL, the cell viability increased by 70.23 and 73.52% respectively compared with the control. Anti-oxidant activity and major chemical component analyses of twenty [22] The assay is based on the reduction of Mo (VI) to Mo (V) by the extract and subsequent formation of green phosphate complex at acid PH. Nitric oxide (NO) is an essential bioregulatory molecule with many physiological functions, such as blood pressure regulation, neural signal transmission, control vasodilatation, smooth muscle relaxation and immune response. [. Free radical theory of aging. PDF Phytochemical screening, free radical scavenging, antioxidant activity Increasing the extraction temperature has been found to enhance the recovery of phenolic compounds as described in previous reports. 2022 May;29(5):3122-3132. doi: 10.1016/j.sjbs.2022.03.015. However, incubation of cells with EEF and BuF remarkably decrease MDA content by 32.01 and 40.29%, compared with hypoxia group. All authors read and approved the final manuscript. Materials and methods: Forty-two patients with TMJ pain were included in this study. Kim DO, Jeong SW, Lee CY. GSH is a naturally occurring substance that is abundant in many living creatures; GSH depletion increases the sensitivity of cells to various aggressions leading to tissue disorder and injury [64]. Values are means of 3 replicates SD; different letters within the same column indicate significant difference at P < 0.05 by Duncans test, The correlation between TPC and TFC, antioxidant activity, and free radical-scavenging capacity of the extracts obtained at different temperatures is interesting to note. After the samples were cooled to room temperature, the absorbance of the aqueous solution of each sample was measured at 695 nm against blank in UV-2450 spectrophotometer (Shimadzu, Japan). Nat Prod Res. For all the extraction temperatures surveyed, the highest TPC, TFC, and antioxidant activities were observed in the extracts obtained at 100C followed by extraction at 90C. Significant correlation was found for TPC and TFC with different extraction temperatures. Antioxidant activity of Tinospora cordifolia leaf extracts through non Al-farsi , Lee CY. At 250 g/ml concentration, the antioxidant activity of the free radical scavenging activities of the extracts assayed through DPPH, reducing power, phosphomolybdenum and metal chelating activity were found to be highest with methanol, followed by ethyl acetate, butanol and water extract. Antimutagenesis and anticarcinogenesis mechanisms II. Gulcin I. Antioxidant activity of food constituents: an overview. The TPC and TFC of extracts have strong positive correlation with the extraction temperature, with the correlation coefficient (R2) 0.9925 and 0.9928, respectively [Figure 2]. Formation of thiobarbituric acid reactive substances from deoxyribose in the presence of iron salts: the role of superoxide and hydroxyl radicals. NS made significant contribution to acquisition of data, analysis, drafting of the manuscript. [. Central inhibitory effects of the methanol extract of Neorautanenia mitis root in rats and mice. FOIA Therefore, Fe2+ can be monitored by absorbance measurement at 700 nm. Maleki S, Seyyednejad SM, Damabi NM, Motamedi H. Antibacterial activity of the fruits of Iranian Torilis leptophylla against some clinical pathogens. Dai S-J, Chen R-D, Yu D-Q. - could be the primary target of antioxidants against oxidative stress [38]. Kim SJ, Matsushita Y, Fukushima K, Aoki D, Yagami S, Yuk HG, et al. Total antioxidant activity by phosphomolybdenum method The total antioxidant capacity of the methanol extract was determined by the phosphomolybdenum method. Springer Protocols Handbooks. Electrochemical approaches employed for sensing the antioxidant capacity exhibited by vegetal extracts: a review. Food Science and Human Wellness, 2014;3(3-4):11016. General speaking, ethyl acetate and n-butanol fractions with higher levels of phenolic, flavonoids content exhibited higher antioxidant ability. The TPC were calculated by using GA calibration curve. Luo H, Huang J, Liao WG, Huang QY, Gao YQ. LDH, which is a stable cytoplasmic enzyme present inside cells, will released into the cell culture supernatant when the cell membrane is damaged [45]. Differences between means were determined using one-way ANOVA and Duncans test. Role of free radicals and catalytic metal ions in human disease: an overview. Treatment with TLM significantly reversed these changes. Antioxidant and antimicrobial activities of crude sorghum extract. Pohanka M. Alzheimers disease and related neurodegenerative disorders: implication and counteracting of melatonin. Thereby enhancing solubility of the polyphenols and inhibiting coagulation with lipoprotein will increase polyphenols yield. The antioxidant activity of fractions was evaluated by phosphomolybdenum method according to the procedure of Prieto et al.. An aliquot of 0.1 ml of each fraction (dissolved in respective solvent) was combined in a vial with 1 ml of reagent solution (0.6 M sulphuric acid, 28 mM sodium phosphate and 4 mM ammonium molybdate). CAS 2015;79:28191. Sahreen S, Khan MR, Khan RA. Keywords: Antioxidant activity, extraction temperature, Gynura divaricata, total phenolic content, total flavonoid content Go to: INTRODUCTION Antiproliferative constituents from, Chen L, Wang JJ, Zhang GG, Song HT, Qin LP. Reactive free radicals, including superoxide, hydroxyl radical, and peroxyl radical, generally result in degradation of protein, lipid peroxidation, and oxidation of DNA, which have been considered to be linked with many chronic diseases, such as diabetes, cancers, and atherosclerosis. The site is secure. Gepdiremen A. Antiradical and antioxidant activity of total . MS participated in the design and collection of data and analysis. However, incubation of cells with EEF and BuF remarkably blocked LDH leakage by 22.12 and 23.60%, respectively, compared with the hypoxia group (Fig. In the present study following findings were observed: (i) Determination of the AOA in different solvent leaf extracts (DSE) of AP and GT by 2, 2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant capacity (TAC) by the phosphomolybdenum method (PM) and 2, 2-azinobis- (3-ethylbenzothiazoline-6-sulphonate) (ABTS) method. the contents by NLM or the National Institutes of Health. Recently, we used single-factor experiments to optimize the extraction conditions of TPC from G. divaricata. italica L.) leaf, stem and flower. Antioxidant activity and free radical-scavenging capacity of The increase in MDA levels in liver suggests enhanced lipid peroxidation leading to tissue damage [63]. The phosphomolybdate method has been used routinely to evaluate the total antioxidant capacity of plant extracts. Vaz JA, Barros L, Martins A, Santos-Buelga C, Vasconcelos MH, Ferreira ICFR. [27,28] Also, increasing extraction temperature will contribute to the release of bound polyphenols in plants with the breakdown of cellular constituents of plant cells which leads to increased cell membrane permeability. Careers. extracts. government site. Chemical investigation and anti hepatotoxic activity of the root bark of Caparis spinos. Phosphomolybdenum assay. Free Radic Biol Med. 10222007 DOI: 10.1006/abio.1999.4019 Abstract A spectrophotometric method has been developed for the quantitative determination of antioxidant capacity. Radical-scavenging-linked antioxidant activities of extracts from black chokeberry and blueberry cultivated in Korea. Effects of EEF and BuF on LDH release (a) and MDA content (b) in PC12 cells under hypoxia condition. Lipid peroxidation is one of the most important mechanisms contributing to oxidative stress. In this paper, to determine whether the protective effects of EEF and BuF on hypoxia-induced injury were mediated by their antioxidant functions, the activities of antioxidant enzymes in PC12 cell under hypoxia with or without pretreatment of EEF and BuF were investigated. . p<0.01 vs. hypoxia. The total antioxidant capacity of the extracts was evaluated according to the method described by Prieto et al. Sharififar F, Dehghn-Nudeh G, Mirtajaldini M. Major flavonoids with antioxidant activity from Teucrium polium L. Khan RA, Khan MR, Sahreen S. Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. PC12 cells are pre-incubated with 200g/ml EEF or BuF for 1h prior to hypoxia for 24h. Data are shown as meanSD (n=6). The TPC in the extracts was determined by using FolinCiocalteus phenol reagent and external calibration with GA and the results were expressed as mg GA/g dry material. The absorbance of the reaction mixture was measured at 350 nm by using UV-2450 spectrophotometer (Shimadzu, Japan). FolinCiocalteu and aluminum chloride colorimetric assay were used to determine the TPC and TFC of Gynura divaricata leaf extracts at different temperatures. Hernansanz-Agustn P, Izquierdo-lvarez A, Snchez-Gmez FJ, Ramos E, Villa-Pia T, Lamas S, et al. Study advance on chemical constituents and pharmacological activities of the Tibeta medicine Dali. The results of the total phenolics content expressed in mg/100 g of gallic acid equivalent (GAE) showed that the n-butanol extract has significantly (p < 0.05) higher phenolics content (381 +/- 1.13) than the methanol and acetone extracts. Total Antioxidant Capacity and Total Oxidant Status of - PubMed Received 2010 Jun 12; Revised 2010 Aug 15; Accepted 2011 Jan 20. CE: Crude extract, HF: n-hexane fraction, EEF: ethyl acetate fraction, BuF: n-butanol fraction, WE: water fraction, Vc: ascorbic acid. Jollow DJ, Mitchell JR, Zampaglione N, Gillete JR. Bromobenzene induced liver necrosis. p<0.01 vs. hypoxia. 2012;26(23):2182-7. doi: 10.1080/14786419.2011.647023. Extraction temperature influences the total phenolic content (TPC), total flavonoid content (TFC) of medicinal plant extracts to a great extend. For the extraction temperatures studied, temperature below 80C showed a significantly lower total phenolic and total flavonoid contents compared with extractions at 90C and 100C. Oluyemi KA, Okwuonu UC, Baxter DG, Oyesola TO. Zheleva-Dimitrova D, Nedialkov P, Kitanov G. Radical scavenging and antioxidant activities of methanolic extracts from Hypericum species growing in Bulgaria. The order of free radical-scavenging capacity of the extracts was 100C > 90C > 80C > 60C > 40C. Phosphomolybdenum method to determination of total antioxidant capacity in the L. Acidissima plant extract. The benzene fraction showed maximum antioxidant activity in all tested assays, namely, phosphomolybdenum, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant capacity (CUPRAC) assays. Previous reports suggested that the reducing properties have been shown to exert antioxidant action by donating of a hydrogen atom to break the free radical chain [44]. The reduce power (a) and phosphomlybdenum assay (b) of crude extract and solvent fractions of R. anthopogonoides. The results of free radical-scavenging capacity of the extracts obtained at different temperatures by DPPH and ABTS methods are shown in Table 2. In this study, 33 male volunteers were exposed to a 1.5 T static magnetic field for a short time and the TAC, TOS and OSI of each subject were determined. Biochem Anal Biochem 1(1):106, Patras A, Brunton NP, Downey G, Rawson A, Warriner K, Gernigon G (2011) Application of principal component and hierarchical cluster analysis to classify fruits and vegetables commonly consumed in Ireland based on in vitro antioxidant activity. In case of blank, 0.5 mL of 45% ethanol was used in place of sample. Food Chem. Epub 2020 Jul 26. Bursal E, Gulcin I. Polyphenol contents and in vitro antioxidant activities of lyophilized aqueous extract of kiwifruit (Actinidia deliciosa). Analysis of Antioxidant Activity Total Antioxidant Activity by Phosphomolybdenum Method. Evaluation of Total Antioxidant Capacity (TAC) by Phosphomolybdenum Method The total antioxidant capacities of PPE and the pure polyphenolic compounds were evaluated by the method of Prieto et al. The optimal extraction temperature of G. divaricata leaf, chosen as a comparison between the yield of phenolic components (TPC and TFC) and their antioxidant and free radical-scavenging capacities (DPPH and ABTS), were 45% ethanol for 30 min at 90C. Asha VV. Saponins are natural products, which have been shown to possess antioxidant properties [65,66]. Antioxidant Capacity and Antimutagenic Potential of BMC Complement Altern Med 14(1):19, CrossRef Nanomaterials (Basel). p<0.01 vs. control. The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1472-6882/12/221/prepub, National Library of Medicine New York: Academic Press; 1990. p. 185. With the ascertained antioxidant activity of this plant, the separation and identification of the antioxidative components in the ethanol extract and using responsesurface methodology (RSM) optimization, the extraction of phenolic compounds should be further investigated. The antioxidant capacity of the crude extract and fractions of R. anthopogonoides was found to decrease in the order n-butanol>crude extract>ethyl acetate>hexane>water (Fig. Source of Support: This project was supported by the graduate student innovation fund of Jiangxi (No.YC10A019), National Library of Medicine The potassium permanganate method generated similar results to the iodimetric method. Comparison of fatty acid profile and antioxidant potential of extracts of seven Citrus rootstock seeds. Evaluation of antioxidant and free radical scavenging capacities of some Nigerian indigenous medicinal plants. In: Lester Packer ANG, editor. The total antioxidant activity was expressed as the absorbance of the sample at 695 nm. ABTS + was produced by reacting the ABTS stock solution with 2.45 mmol/L potassium persulfate (final concentration) and allowing the mixture to stand in the dark at room temperature for 1216 h before use.
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